Peptide Dilution and Serial Dilution Calculator

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NOVABIO · LABORATORY RESEARCH TOOL

Peptide Dilution and Serial Dilution Calculator

Use C1V1=C2V2 for a single dilution or build a serial dilution table from an initial concentration, per-step factor, number of steps and final tube volume.

Single-step C1V1=C2V2Up to 24 serial stepsTransfer and diluent table
DIRECT ANSWER

Use C1V1=C2V2 for a single peptide dilution or generate a serial dilution table with transfer volume, diluent volume and concentration by step.

Plan a single dilution

Stock and target concentrations must belong to the same dimension. The target may equal, but cannot exceed, the stock.

Build a serial dilution table

Enter the same final volume for every tube. The planner calculates one transfer volume and one diluent volume per step.

For a 1:10 step, enter 10.
Common factors

FORMULA & HOW IT WORKS

Peptide Dilution Calculator: calculation method

Each result shows the governing relationship and keeps unit normalization separate from final display formatting.

Single dilution

The stock volume is solved from the standard conservation relationship C1V1=C2V2.

V1 = C2 × V2 ÷ C1

Diluent volume

Diluent is the difference between final volume and calculated stock volume.

Diluent = V2 − V1

Serial concentration

Each step divides the prior concentration by the selected per-step factor.

Cn = C0 ÷ dilution factorⁿ

Per-step transfer

For equal final volumes, the transfer equals final tube volume divided by the step factor.

Transfer = final tube volume ÷ step factor

WORKED LABORATORY EXAMPLE

Worked 1:10 serial dilution

Calculation steps

  1. Initial concentration is 100 µg/mL.
  2. Per-step dilution factor is 10 and final volume is 1 mL per tube.
  3. Each step uses 0.1 mL transfer plus 0.9 mL diluent.
  4. After three steps, the theoretical concentration is 0.1 µg/mL.

How to interpret it

This ideal calculation assumes complete mixing and exact volumetric transfer at each step. Laboratory uncertainty compounds across serial steps.

INPUT & UNIT DEFINITIONS

Understand every variable before calculating

The labels below define the mathematical inputs used on this page. They are not preparation instructions.

C1

Initial or stock concentration.

V1

Volume of stock required.

C2

Target concentration after dilution.

V2

Final completed volume.

Dilution factor

The ratio between the concentration before and after one dilution step.

Cumulative factor

The product of all per-step dilution factors through the selected step.

LIMITATIONS

What this calculator does not determine

The planner assumes ideal volume additivity, complete mixing and exact transfer. It does not correct for pipette tolerance, retained volume, evaporation, adsorption, matrix effects or uncertainty propagation. A target concentration above the entered stock concentration is rejected because dilution cannot increase concentration.

FREQUENTLY ASKED QUESTIONS

Dilution & Serial Dilution FAQ

What does C1V1=C2V2 calculate?

It relates stock concentration and stock volume to target concentration and final volume for a single ideal dilution.

Why is a target above stock concentration rejected?

Adding diluent cannot increase concentration. A higher target requires a different stock or a concentration process.

How is diluent volume calculated?

The calculated stock volume is subtracted from the selected final volume.

What does a 1:10 serial dilution mean here?

A dilution factor of 10 means each step contains one part transferred material within ten parts final volume.

Why can serial dilution error accumulate?

Every transfer and volume measurement has uncertainty, and those uncertainties can propagate through later steps.

Can mass concentration and molar concentration be mixed?

No. Stock and target units must belong to the same concentration dimension unless a separate molecular-weight conversion is performed first.

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METHODOLOGY

Transparent units and unrounded intermediate values

The shared NOVABIO calculation engine converts inputs to SI-aligned base units, applies the displayed formula and converts the unrounded result into the selected display unit. Invalid, zero, negative and dimensionally incompatible inputs are rejected. Last reviewed: October 6, 2026.
Research-use notice: This tool performs laboratory mathematics from user-entered values. It does not verify a product, select a target amount, recommend a dose, provide an injection protocol or determine whether any material is suitable for human or veterinary use.